Guest lecture by Maria Jassinskaja | Intra- and extracellular proteome dynamics in blood progenitor cells during development, malignant transformation and ex vivo expansion

On 26 September 2024 (2:00 PM) Maria Jassinskaja PhD (Dept of Biology, University of York, UK) will give a guest lecture at Sanquin, Amsterdam.

Title: Intra- and extracellular proteome dynamics in blood progenitor cells during development, malignant transformation and ex vivo expansion

Host: Arthur Svendsen

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Abstract
Delineating cell-intrinsic and -extrinsic regulators of hematopoietic stem and progenitor cell (HSPC) function is critical to improve efforts in ex vivo hematopoietic stem cell (HSC) expansion and to better understand leukemia cell biology. While numerous tools have now been developed to study the genome and transcriptome at single cell resolution, multiple biological contexts are associated with a disconnect between mRNA levels and the abundance of the functional units of the cells – the proteins. To bridge this gap, we have over the last several years optimized and applied an array of mass spectrometry (MS)-based approaches to study the molecular makeup of HSPCs and their environment (Jassinskaja et al., 2017; Jassinskaja et al., 2021; Pimkova et al., 2022; Jassinskaja, Ghosh et al., 2024) In this seminar, I would like to share the biological insights we have gained by applying these approaches to HSPCs in the context of development, clonal hematopoiesis, and leukemia, as well as how we have utilized an ex vivo HSC expansion platform to directly couple the composition of the cellular and secreted proteome to in vivo function of blood stem and progenitor cells.