Kits

Sanquin’s MAT kit is a highly sensitive in vitro human cell-based assay for pyrogen detection, worldwide available through our partner Lonza. The European Pharmacopeia (EP) states that, after a product-specific validation, the Monocyte Activation Test (MAT) is suitable as a replacement for the rabbit pyrogen test.

The MAT Cell Set offers a pool of cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) with dedicated Culture Medium Supplement ensuring a high sensitivity.

Test procedure

Day 1:
Under non-pyrogenic conditions:

• To a 96-wells plate add culture medium, endotoxin standard, product samples & MAT Cells
• Incubate for 18-24h at 37ºC

Day 2 (ELISA):
Under standard lab conditions:

• Transfer supernatant into cytokine ELISA
• Measure cytokine levels and analyze samples

Sanquin products for MAT

Day 1 (Cell incubation):

MAT Cell Set HS (M2017) 

• 3 vials of cryopreserved pooled PBMCs
• 3 vials of MAT Human Serum Supplement
• Fully compliant with Ph. Eur. chapter 2.6.30. requirements
• Limit of Detection (LOD) for endotoxin ≤ 0.08 EU/mL*
• Improved detection of Non-Endotoxin Pyrogens
   

MAT Cell Set (M2016)

• 3 vials of cryopreserved pooled PBMCs
• 3 vials of dedicated MAT Culture Medium Supplement (based on FBS)
• PMBCs qualified according to Ph. Eur. chapter 2.6.30
• Limit of Detection (LOD) for endoxtoxin ≤ 0.02 EU/mL
• Detection of Non-Endotoxin Pyrogens

Day 2 (ELISA):

PeliKine Human IL-6 ELISA Rapid Set A (M2018) [NEW]
PeliKine Human IL-6 ELISA Rapid Set B (M2019) [NEW]

• Highly sensitive IL-6 ELISA sufficient for three 96-well plates
• IL-6 standard range (0.6 – 450 pg/mL)
• Limit of Detection (LOD): 1 pg/mL (static incubation)

Why use Sanquin’s MAT kit?

• Qualified according to European Pharmacopoeia Chapter 2.6.30
• High sensitivity 
• Reactive towards non-endotoxin pyrogens
• Measurement of IL-6 as cytokine read-out
• Proven robustness and reliability
• Storage at -80ºC (no liquid nitrogen required)
• Assay time 24 hours including overnight culture
• MAT cell Set HS (M2017) further reduces the use of animal-derived components

Why cryopreserved pooled PBMC as cell source?

• Comparable reactivity to fresh PBMC
• No need for blood donors and cell isolation for each experiment
• Pool of 4 donors taking donor variation into account
• Available on demand
• Storage at -80ºC (no liquid nitrogen required)
• Proven robustness and reliability
   

Why IL-6 as cytokine read-out?

• High sensitivity
• Fully secreted into the medium
• Stable in (frozen) supernatant

Interested? Please mail to: [email protected]

Hesitant to perform the assay in your own lab? Check our MAT services.

*Concentration stated as measured in the sample.