Thesis defense Iris Kreft

On 20 November 2024 Sanquin researcher Iris Kreft defended her thesis 'Profiles in Spectra: Mass spectrometry-based proteomics of plasma and platelets in hematological disorders' at Utrecht University.

Promotor
Prof AB Meijer PhD

Co-promotor
M van den Biggelaar PhD

Venue
Academy Building, Utrecht University 

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Abstract
Proteins are key regulators of biological processes and variations in their quantity and quality are related to disease. In this thesis, we use state-of-the-art mass spectrometry (MS) to demonstrate how protein profiling can provide insights into hematological disorders.  
 
First, we studied variation in plasma protein levels in healthy blood donors, before, during and after whole blood donation. This showed the ability of MS to identify and quantify plasma proteins and plasma protein complexes that reflect the functionality of platelets, red blood cells and immune cells.  
 
Next, we investigated plasma profiles of patients with hematological disorders, including acute myeloid leukemia, graft-versus-host disease, and thrombosis. Here, we discovered specific protein changes related to inflammatory responses and found that protein levels in patients at risk of thrombosis could be linked to the underlying disease or treatment. 
 
In children with inherited platelet disorders, we mapped the platelet proteome to unravel the effects of genetic mutations on protein profiles. We discovered that mutations in cytoskeleton-related genes have a significant impact on platelet proteomes and constructed a disease map which enabled identification of both known as well as novel proteins associated with alpha-granules. This study enhances the understanding of genotype-phenotype relationships and provides opportunities to investigate bleeding disorders of unknown cause. 
 
Finally, we developed a MS method for the identification and quantification of benign polymorphisms and pathogenic variants of the adhesive glycoprotein Von Willebrand Factor. We showed that MS can be used as a diagnostic assay in patients with Von Willebrand disease to support disease classification.